DBCO-PEG-SH DBCO聚乙二醇巯基 修饰性衍生物 Ponsure

产品名称：   DBCO PEG巯基   DBCO-PEG-SH

商品型号：   PS2-SDB

英文名：     DBCO-PEG-SH

数量：     现货或一周

保质期：    一年以上

保存条件     -20℃

规格：      1g、5g、10g，大包装另寻

详细信息：

取代率：90%以上

分散系数：小于等于1.05

常规分子量：2K、3.4K、5K

结构式：

物理性质：

状态：淡黄色到白色固体，或者半固体，取决于分子量。

溶解性：溶于水，CH2Cl2,DMSO,DMF等溶剂。

保持条件：-20℃长期保存，避光，干燥。

注意事项：取用一定要干燥，避免频繁的溶解和冻干。

应用简介：

DBCO (dibenzocycolctyne) PEG thiol (DBCO-PEG-SH) derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Copper-free Click Reaction Procedures:

Prepare the azide-containing reagents in reaction buffer.

Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.

Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4℃.

Purify conjugates either by dialysis or size-exclusion chromatograph.

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DBCO-PEG-SH DBCO聚乙二醇巯基 修饰性衍生物 Ponsure